调制叶绿素荧光成像系统

主要功能

• 成像功能：对 Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等至少 17 种参数进行成像分析。测定调节性能量耗散 Y(NPQ)，反映植物光保护能力，测定非调节性能量耗散Y(NO)，反映植物光损伤程度。

• 程序测量功能：可程序测量荧光诱导曲线、快速光曲线和暗弛豫，也可手动测量；在测量过程中能自动分析所有荧光参数的变化趋势

• AOI 功能：可在测量前或测量后任意选择感兴趣的区域（AOI），程序将自动对选择的 AOI 的数据进行变化趋势分析，并在报告文件中显示相关 AOI 的数据。所有报告文件中显示的数据都可导出到 EXCEL 文件中。

• 成像异质性分析功能：对任意参数任意时间的成像，可在图像上任意选取两点，软件自动对两点间的数据进行横向异质性分析，并可导出到 EXCEL 文件中。

• 成像数据范围分析功能：对任意参数任意时间的成像，可分析任意两个荧光数值之间有多少个像素点，多少面积（cm²）。

• 突变株筛选功能：可跟据成像结果快速筛选光合、产氢/油、抗逆（抗盐、抗旱、抗病等）等突变株。

• 微藻毒理研究功能：可同时测量 96 个微藻样品（对照和处理组）的光合活性，软件自动给出处理组样品相对于对照组的光合抑制百分比。

• 吸光系数测量功能：快速测量叶片的吸光系数。吸光系数测量光源： 16 个红光（650 nm）和 16 个近红外（780 nm）LED，用于测量植物叶片或藻类样品 PAR 吸光系数。

测量参数

Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

应用领域

• 光合作用研究：可以在完全相同的条件下同时对大量样品进行成像

• 植物病理学：病斑部位（包括肉眼不可见时）成像以及病斑扩散的时空动力学

• 植物胁迫生 理学：肉眼不可见胁迫损伤的早期检测

• 遗传育种：出苗后大规模快速筛选高光合/抗旱/抗热/抗冻/抗病等植株

• 突变株筛选：快速筛选模式植物的光合突变株、抗逆突变株、产氢微藻突变株等

• 微藻毒理学：不同毒物浓度多个重复的样品一次测完，软件自动计算抑制比率

• 分子生物学：宏观水平上检测样品的绿色荧光蛋白（GFP）荧光

• 其它多种扩展研究

选购指南

一、荧光成像 MAXI 版

系统组成：通用型主机，LED 供电单元， LED 光源，CCD，数据线，软件等。

注意：高等植物或真核藻类测量时选择蓝光光源（推荐），蓝藻测量请选择红光光源。

二、荧光成像 MINI 版

系统组成：通用型主机（下图中未显示），LED 光源（如下图），CCD，数据线，软件等。

注意：高等植物或真核藻类测量时选择蓝光光源（推荐），蓝藻测量请选择红光光源。

三、荧光成像 MICROSCOPY 版

系统组成：通用型主机，特制显微镜，CCD，数据线，软件等。

产地：德国 WALZ

数据来源：光合作用文献 Endnote 数据库，更新至 2021 年 1 月，文献数量超过 10000 篇

原始数据来源：Google Scholar

Chen, Y., et al. (2021). "Low UVA intensity during cultivation improves the lettuce shelf-life, an effect that is not sustained at higher intensity." Postharvest Biology and Technology 172: 111376.

Grimmer, J., et al. (2020). "Mild proteasomal stress improves photosynthetic performance in Arabidopsis chloroplasts." Nature communications 11(1): 1662.

García-Cerdán, J. G., et al. (2020). "Chloroplast Sec14-like 1 (CPSFL1) is essential for normal chloroplast development and affects carotenoid accumulation in <em>Chlamydomonas</em&gt." PNAS: 201916948.

Garcia-Molina, A. and D. Leister (2020). "Accelerated relaxation of photoprotection impairs biomass accumulation in Arabidopsis." Nature Plants.

Amstutz, C. L., et al. (2020). "An atypical short-chain dehydrogenase–reductase functions in the relaxation of photoprotective qH in Arabidopsis." Nature Plants 6(2): 154-166.

Acebron, K., et al. (2020). "Diurnal dynamics of non-photochemical quenching in Arabidopsis npq mutants assessed by solar-induced fluorescence and reflectance measurements in the field." New Phytologist n/a(n/a).

Swift, T. A., et al. (2020). "Photosynthesis and crop productivity are enhanced by glucose-functionalized carbon dots." New Phytologist n/a(n/a).

Duan, L., et al. (2020). "Characterization of CYCLOPHILLIN38 shows that a photosynthesis-derived systemic signal controls lateral root emergence." Plant Physiology.

Adamakis, I.-D., et al. (2020). "Hydrogen Peroxide Production by the Spot-Like Mode Action of Bisphenol A." Frontiers in Plant Science 11.

Adamakis, I. S., et al. (2020). "Rapid Hormetic Responses of Photosystem II Photochemistry to Cadmium Exposure." anatomy & morphology.

Andrzejczak, O. A., et al. (2020). "The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming. ." Int. J. Mol. Sci(21): 1546.

Araniti, F., et al. (2020). "Metabolomic, proteomic and physiological insights into the potential mode of action of thymol, a phytotoxic natural monoterpenoid phenol." Plant Physiology and Biochemistry.

Ås Hovind, A. B., et al. (2020). "Functional trade-off of hydration strategies in old forest epiphytic cephalolichens." Fungal Biology.

Bednarczyk, D., et al. (2020). "Influence of short-term exposure to high light on photosynthesis and proteins involved in photo-protective processes in tomato leaves." Environmental and Experimental Botany 179: 104198.

Begum, N., et al. (2020). "AMF inoculation and phosphorus supplementation alleviates drought induced growth and photosynthetic decline in Nicotiana tabacum by up-regulating antioxidant metabolism and osmolyte accumulation." Environmental and Experimental Botany: 104088.

Borlongan, I. A., et al. (2020). "The effects of temperature and irradiance on the photosynthesis of two heteromorphic life history stages of Saccharina japonica (Laminariales) from Japan." Journal of Applied Phycology.

Chen, Z., et al. (2020). "Functional growth, photosynthesis and nutritional property analyses of lettuce grown under different temperature and light intensity." The Journal of Horticultural Science and Biotechnology: 1-9.

Chen, Z., et al. (2020). "Comprehensive analysis of the Ppatg3 mutant reveals that autophagy plays important roles in gametophore senescence in Physcomitrella patens." BMC Plant Biology 20(1): 440.

Claquin, P., et al. (2020). "Singular physiological behavior of the scleractinian coral Porites astreoides in the dark phase." Coral Reefs.

Correia, P. M. P., et al. (2020). "Photoprotection and optimization of sucrose usage contribute to faster recovery of photosynthesis after water deficit at high temperatures in wheat." Physiologia plantarum n/a(n/a).

Cruz de Carvalho, R., et al. (2020). "Using Chlorophyll a Fluorescence Imaging to Select Desiccation-Tolerant Native Moss Species for Water- Sustainable Green Roofs." Water 12.

Fan, Z.-q., et al. (2020). "Involvement of BrNAC041 in ABA-GA antagonism in the leaf senescence of Chinese flowering cabbage." Postharvest Biology and Technology 168: 111254.

Feng, S., et al. (2020). "Morphological and physiological responses of two willow species from different habitats to salt stress." Scientific Reports 10(1): 18228.

Ferreira, T. M. M., et al. (2020). "Effect of salinity stress in Setaria viridis (L.) P. Beauv. accession A10.1 during seed germination and plant development .Ciência e Agrotecnologia." Ciência e Agrotecnologia 44.

Gao, S., et al. (2020). "Photosynthetic characteristics and chloroplast ultrastructure of welsh onion (Allium fistulosum L.) grown under different LED wavelengths." BMC Plant Biology 20(1): 78.

Gauslaa, Y., et al. (2020). "Growth rates and thallus loss in hair lichens along small-scale Picea abies-canopy gradients." Fungal Ecology 47: 100947.

Gómez-Espinoza, O., et al. (2020). "Decomposition of Calcium Oxalate Crystals in Colobanthus quitensis under CO2 Limiting Conditions." Plants(9): 1307.

Ahammed, G. J., et al. (2020). "Overexpression of tomato RING E3 ubiquitin ligase gene SlRING1 confers cadmium tolerance by attenuating cadmium accumulation and oxidative stress." Physiologia plantarum n/a(n/a).

He, J., et al. (2020). "Drought does not induce crassulacean acid metabolism (CAM) but regulates photosynthesis and enhances nutritional quality of Mesembryanthemum crystallinum." PLoS ONE 15(3): e0229897.

Ho, L. H., et al. (2020). "Potassium Application Boosts Photosynthesis and Sorbitol Biosynthesis and Accelerates Cold Acclimation of Common Plantain (Plantago major L.)." Plants (Basel) 9(10).

Huang, J., et al. (2020). "CaASR1 promotes SA- but represses JA-dependent signaling to enhance resistance of Capsicum annuum to bacterial wilt by modulating CabZIP63." Journal of Experimental Botany.

Huizhen, D., et al. (2020). "Genome-wide Identification of PbrbHLH Family Genes, and Expression Analysis in Response to drought and Cold Stresses in Pear (Pyrus Bretschneideri)." BMC Plant Biology.

Jaghdani, S. J., et al. (2020). "Mg deficiency induces photo-oxidative stress primarily by limiting CO2 assimilation and not by limiting photosynthetic light utilization." Plant Science: 110751.

Jiang, X., et al. (2020). "Light-induced HY5 Functions as a Systemic Signal to Coordinate the Photoprotective Response to Light Fluctuation." Plant Physiology 184(2): 1181-1193.

Jurado-Flores, A., et al. (2020). "Exploring the Functional Relationship between y-Type Thioredoxins and 2-Cys Peroxiredoxins in Arabidopsis Chloroplasts." Antioxidants (Basel) 9(11).

Kaiser, E., et al. (2020). "Growth under Fluctuating Light Reveals Large Trait Variation in a Panel of Arabidopsis Accessions." Plants (Basel) 9(3): 316.

Kulikova, N., et al. (2020). "Silver nanoparticles stabilized by humic substances adversely affect wheat plants and soil." Journal of Nanoparticle Research 22.

Lan, C.-Y., et al. (2020). "Comparisons of Chlorophyll Fluorescence and Physiological Characteristics of Wheat Seedlings Influenced by Iso-Osmotic Stresses from Polyethylene Glycol and Sodium Chloride." Agronomy 10: 325.

Li, P., et al. (2020). "Response of lipid productivity to photosynthesis of Chlorella vulgaris under various nutrient stress modes." 12(5): 056102.

Li, X., et al. (2020). "Role of jasmonate in Lolium perenne compensatory growth and photosynthesis: uncoupling with photosynthesis and differential effects on growth." Acta Physiologiae Plantarum 42(6): 95.

Li, X., et al. (2020). "Light Signaling-Dependent Regulation of Photosystem II Biogenesis and Functional Maintenance." Plant Physiology: pp.00200.02020.

Liu, G., et al. (2020). "GREEN STRIPE, encoding methylated TOMATO AGAMOUS-LIKE 1, regulates chloroplast development and chlorophyll synthesis in fruit." New Phytologist n/a(n/a).

Liu, X., et al. (2020). "Ofloxacin induces etiolation in Welsh onion leaves." Chemosphere: 128918.

Llorente, B., et al. (2020). "Synthetic conversion of leaf chloroplasts into carotenoid-rich plastids reveals mechanistic basis of natural chromoplast development." PNAS 117(35): 21796-21803.

Mishra, M., et al. (2020). "How do rice seedlings of landrace Pokkali survive in saline fields after transplantation? Physiology, biochemistry, and photosynthesis." Photosynthesis Research.

Perera-Castro, A. V., et al. (2020). "What drives photosynthesis during desiccation? Mosses and other outliers from the photosynthesis-elasticity trade-off." Journal of Experimental Botany.